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KMID : 0380420020260030207
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Journal of Prventive Veterinary Medicine
2002 Volume.26 No. 3 p.207 ~ p.212
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Proportion of Retinyl Palmitate to Fluorophore of Lipid Extract from Tissues and Liver Subcellular Organelles
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Abstract
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This study was carried out to investigate the age-related changes of the fluorescent intensity and retinyl palmitate concentration in lipid extracts of various organs such as liver, heart, kidney, spleen, and hepatic subcellular organelles like mitochondria and microsome from Sprague Dawley rats that were raised with manually manufactured feeding until 7 month old and sacrificed every month. We compared not only their fluorescence spectra to find out the characteristics of fluorescence from the tissues but also those to assure the effect on retinyl palmitate. The fluorescent intensity from each tissue steadily increased until 4 months. Increment of the fluorescent intensity from each tissue except liver was much lower than that from liver and also was not age-related. Increase of retinyl palmitate concentration in liver was age-dependent and had almost the same figure as that of the fluorescent intensity in liver. Only the liver´s fluorescence spectrum among various organs was similar to retinyl palmitate´ spectrum, which means that the accumulated retinyl palmitate in liver had a big effect. On the other hand, the fluorescence spectra from other tissues had nothing to do with retinyl palmitate due to tissue peroxidation. The proportion of retinyl palmitate to total fluorescence spectrum in liver´s lipid extracts showed age-related increase until 4 months old but had no significant change thereafter. Hepatic subcellular organelles such as mitochondria and microsomes showed the same tendency. Therfore, these results indicate that retinyl palmitate has an effect not only on liver but also on hepatic subcellular organelles.
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